Second Nature Care Blog

India, Covid and Ozone Therapies

[fa icon="calendar'] Oct 23, 2021 10:28:37 AM / by Dr. Isadora Guggenheim posted in Ozone and Prolozone therapies, Chronic Illness and Disease

[fa icon="comment"] 0 Comments

We offer I.V. Ozone for chronic infections, long hauler syndrome and post vax protocols. 


“The promotion of ozone for the improvement of human life is our motto”. 
This is supported by the Bisleri Charitable Trust which in turn promoted the Ozone Forum of India (OFI), an association of doctors that teaches, practices and disseminates ozone therapy as part of economically integrated complementary medicine. 
Ozone therapy has been practiced by the OFI to treat different pathologies including tuberculosis, some forms of cancer, pain therapy, Covid and post Covid. 
To treat patients affected by Covid and tuberculosis, ozone therapy has also been successfully used in hospitals: Shewri Government Hospital for Tuberculosis, the LG Hospital in Ahmedabad and the Sion Hospital in Mumbai.
More than two thousand doctors have been informed and introduced to the use of ozone therapy as part of 83 training programs organized by the OFI.
The projects of the OFI and the Bisleri Charitable Trust are supported by Bisleri International Pvt Ltd (, the largest Indian company for the production and distribution of mineral waters.
Bisleri International Pvt Ltd which treats all its mineral waters with ozone, covers more than 65% of the beverage market in India, has 122 production plants, 4500 distribution centers and 5000 heavy vehicles (trucks and lorries) for to bring mineral water to every area of India.
To better understand the practice, research developments and clinical results in the use of ozone therapy,  Orbisphera interviewed Dr. Mili Shah President of the Ozone Forum of India.
How and when did you find out about ozone therapy and Ozone Forum of India?
In 2005 I came across Ozone Forum of India who was promoting ozone therapy in India.  I have attended ozone therapy training by different doctors from India, Cuba, Russia, Germany & USA.
Ozone Forum of India (OFI) is an integral part of Bisleri Charitable Trust began promoting ozone therapy since 2002, first few years were spent in gathering scientific knowledge from all different countries where ozone therapy was very advanced and were very high on research. OFI was established in 2005 with an intention to provide structured and organized workshops and other public initiatives to spread this low cost therapy and easy access to Ozone therapy all over India. Further, OFI is the training partner for Ministry of AYUSH, and National Institute of Naturopathy, Government of India, for its certification course on Ozone therapy. OFI has started extensive work on tuberculosis, integrated oncology and pain management.  It has published research paper of ozone therapy in different disease. OFI worked extensively on COVID-19 by launching two CTRI studies on ozone therapy as supportive therapy in COVID-19. Recently OFI along with NIN have submitted phase 3 trial and registry studies to interdisplinary committee in AYUSH department to use ozone therapy extensively in Covid 19.
What convinced you to practice ozone therapy?
I instantly took liking towards this oxygen based therapy as it had practically no side effects and was very scientific.  It was originated in Germany and as a practicing homoeopath it had a similar philosophy of treatment.
Over the past 18 months you and Ozone Forum of India have used ozone therapy to prevent and treat patients affected by Covid - 19. Can you provide us with the results of clinical trials carried out on Bisleri employees and in hospitals where you have treated Covid patients with ozone therapy?
This was done in the past during lockdown which improved Bisleri employees’ immunity and well-being. With this treatment, we not only reduced their risk towards contracting the infection but build their immunity which led to only 7 out of 320 employees contract the virus and they were mild and asymptomatic. This also shows the incidence rate off contracting the infection is only 2%.
We have used ozone therapy in Covid, in Government and private hospitals.  In mid 2020, we have conducted 2 CTRI (Clinical Trial Registry of India) which are successfully published in Journal of Immuno Pharmacology (Pub Med Index Journal).  2 more research papers have been published as preventive tool for covid-19.
As per clinical trial conducted in mid-2020 ozone therapy showed recovery rate of 77% within 5 days and by the 8th day all the patients from ozone group recovered. Ozone therapy has a greater impact in controlling the infection as patient suffers from various conditions such as circulatory disorders leading to clotting or thrombosis, headaches, lung infection, body pain & breathlessness situations. We have given ozone therapy to more than 700 patients in Mumbai and Pune.
How much is ozone therapy understood and practiced in India?
Ozone therapy has come a long way from unknown therapy to acceptable complimentary therapy because of our initiatives. Patients by enlarge have started having understanding for this wonderful supportive therapy. We are trying to create more awareness on this with the help of regular interviews, newspaper articles and social media. 
The Ozone Forum of India carries out training courses to know and practice ozone therapy, can you give us an idea of how many ozone therapists have attended your courses and which are the countries where ozone therapy is practiced?
We have trained more than 1900 doctors across India, out of which approx. 750 doctors are practicing Ozone Therapy.
We have trained doctors from UAE, Malaysia, Indonesia, Bangladesh, Srilanka, etc. 
Have you communicated the results of your medical studies and practices regarding ozone therapy to the Ministry of Health and the World Health Organization?
We have communicated results of ozone therapy with our Government and have applied for registry trial on ozone therapy. Meanwhile, lot of ozone practicing doctors have been using ozone therapy on their patients.
What do you think is the future of ozone therapy?
Future of ozone therapy is extremely bright as slowly we are gathering lot of information on how ozone precisely works on human body along with that there are lot of countries which are practicing and regularizing ozone therapy. This is being adjuvant and complimentary therapy, having good potential as integrated in any medical field.
Interview by Antonio Gaspari
Director Orbisphera
For a more in-depth knowledge of the healing abilities of ozone, we recommend the book:
Antonio Gaspari, “Ozone: a cure for life”
Read More [fa icon=long-arrow-right"]

IVERMECTIN - A Miracle Med for Parasitic Infections, Viruses and Inflammation

[fa icon="calendar'] Sep 29, 2021 8:00:00 AM / by Dr. Isadora Guggenheim posted in The Scoop on Poop, Autoimmune Disease, Ozone and Prolozone therapies, Chronic Illness and Disease

[fa icon="comment"] 0 Comments

Anti-Parasite, Anti-Viral and Anti-Inflammatory 

Read More [fa icon=long-arrow-right"]


[fa icon="calendar'] Sep 20, 2021 6:57:46 PM / by Dr. Isadora Guggenheim posted in Ozone and Prolozone therapies, Healthy Living, Chronic Illness and Disease

[fa icon="comment"] 0 Comments

The adoption of oxygen ozone therapy to treat patients severely affected by Covid, as a medical practice to be used for compassionate use, has become the subject of public debate in Spain. 
Read More [fa icon=long-arrow-right"]

OZONE For Life - Acute Chronic Viral Infections

[fa icon="calendar'] Sep 17, 2021 11:29:18 AM / by Dr. Isadora Guggenheim posted in Ozone and Prolozone therapies

[fa icon="comment"] 0 Comments



The doctors of the Covid department of the Valduce Hospital in Como, Italy, had used all the means and therapies available to them. The situation was desperate. For the 63-year-old Mrs. Maria Ausilia, called “Usi”, there seemed to be little chance of recovery. The former husband chartered accountant Francesco Nessi together with the doctors prof. Marianno Franzini, International President of the Scientific Society of Oxygen/Ozone Therapy (SIOOT), and the surgeon Emanuele Franchi were ready for one last therapeutic attempt with oxygen/ozone therapy. 
Dr. Franchi is an orthopedics and traumatology specialist, current Head of the Orthopedics Unit of the Clinical Institute of Villa Aprica in Como, a member of SIOOT, who has been practicing ozone therapy since 2006. 
Interviewed by “Orbisphera”, Dr. Franchi said:
«The SIOOT and prof. Franzini were contacted by dr. Nessi, desperate for the condition of his ex-wife, who after more than two weeks of intensive care intubated and subsequently transferred to the pulmonology ward where she was supported with non-invasive ventilation 24 hours a day, did not seem to improve despite the various therapies reserved for her.  Dr. Nessi was determined to try one last therapy for his wife, so he asked the hospital managers to be able to practice oxygen-ozone therapy under conditions of compassionate protocol. Once he became aware of the lady’s conditions and made an agreement with the hospital for the practice of this therapy, prof. Franzini involved me as an ozone therapist who carries out his own business in Como.
Having dealt with the necessary paperwork, I entered the department on the morning of Saturday 10 April.
The patient was in very serious condition, with the ratio between ventilation perfusion and oxygenation the so-called (P/F) equal to 90. A very low value considered as a critical limit for intubation.
After the interview with the patient’s colleagues, it was immediately clear to me that despite the therapies used up to that time and the long resuscitation treatment, the patient’s clinical condition did not improve and, after a careful evaluation, it was considered unlikely that the physique of the lady already strongly weakened could withstand other invasive treatments such as resuscitation maneuvers.
As foreseen by the protocol studied with prof. Franzini, we therefore started with the first major autohemotherapy (MAH), which was then repeated in the evening.  Oviously, during the whole period in which we practiced oxygen-ozone therapy, none of the original therapies in progress were suspended.
The surprising thing is that on Sunday morning, after just two MAH sessions, the ratio (P/F) between ventilation perfusion and oxygenation had already risen to 210. This was a sensational response.
In the following days the parameters of (P/F) stabilized between 270 and 300, an exceptional result if you consider that we started from 90.
Even knowing how effective MAH was, I was the first to be amazed at how sensational the effect of ozone therapy had been on the lady’s health conditions.
We continued with this treatment for four days.
In the following days, we witnessed a constant improvement in clinical and laboratory parameters.
The values of the C-reactive protein that measures inflammation fell from 150 to 40,
and the values of the D-dimer which measures the coagulation mechanisms related to the formation of thrombi fell from 1500 to 500.
After a few days of therapy and clinical improvement, a control CT scan of the chest was repeated which surprisingly showed a marked improvement in the pulmonary infiltrative foci.
We therefore decided to continue with the treatment once a day for about another week.
A few days after discontinuing the therapy, Dr. Nessi reported that his wife was fine and even managed to celebrate her birthday a few days after the end of the therapy with the staff by going out on the terrace of her hospital room.
I am an orthopedics specialist and I find it difficult to understand all the mechanisms of action of ozone, but by evaluating the clinical parameters that I am able to understand, I can say that the improvement seems surprising to me.
It is the first time that I have personally treated a Covid-19 patient with ozone therapy and it seems to me an example of remarkable success in the ability to cure.
The results are consistent and in line with what has been observed and published in the case reports and in the international bibliography in which 890 Covid-19 patients are successfully treated with the aid of oxygen-ozone therapy associated with standard therapies used in dedicated departments.
What amazed me most is that Covid-19 patients treated with oxygen ozone therapy in combination with conventional therapies reported in the literature, were subjected to protocols that provided for the use of this treatment developed for patients in less dire conditions. 
This, in my opinion, suggests that if we had intervened at the first symptoms rather than when the picture was so impaired, the course of the lady’s illness might have been more favorable from the beginning.
After the oxygen ozone therapy, the patient tested negative for Covid-19, so it would be worth furthering the studies to understand if and to what extent this therapy, which is known to have antibacterial and antiviral properties, may have contributed to this result.
I am fascinated and favorably surprised by the behavior of ozone, even though I have long verified its anti-inflammatory and anti-infective capacity in patients I have treated with bacterial loads that could not be eradicated with normal drug treatment.
I was very confident in ozone’s ability to fight Covid, however I was amazed by the results that went far beyond my expectations.
Unfortunately, despite the results obtained in this and in other cases reported in the literature, to date an appropriate experimentation and evaluation process has not been developed that allows oxygen-ozone therapy to be recognized as an adjuvant to those known to all and used in the Covid-19 disease therapy more universally.
Reflecting on what happened in my limited experience and comparing myself with some fellow ozone therapists who have been involved in the treatment of other patients suffering from Covid-19 disease, I am convinced that it would be worth putting the effort into carrying out a comprehensive research aimed at “approval of oxygen-ozone therapy as a hospital protocol for the treatment of Covid-19 patients”. I also hope that other hospitals may consider in the interim the use of therapy as a compassionate cure for those cases for which the standard therapies recognized and used on a large scale have unfortunately not brought satisfactory results, in the hope that other patients can draw the benefit that I have been able to observe.
If further studies and the collection of other cases confirm the validity of oxygen-ozone therapy as an adjuvant to standard care, oxygen-ozone therapy could help save more lives and would also benefit hospitals dedicated to the care of Covid-19 positive patients, especially in terms of patient recovery speed».
It is also worth noting that all the interventions made by dr. Franchi for practicing oxygen ozone therapy were done completely free of charge.
The costs of using the machines and bags to carry out the MAH were also covered free of charge by SIOOT.
Concluded Dr. Franchi: «The compassionate protocol provides for free treatment and in any case my service aimed to heal the lady regardless of the costs».
Thanks, Dr. Franchi, Prof. Franzini and Dr. Nessi.  Your interventions not only contributed to saving a person, and as the Talmud says “whoever saves a life saves the whole world”, and this action has fueled the hope of assisting in the best way the other therapies already recognized and proven to defeat Covid and other viruses to come.
Antonio Gaspari
Director Orbisphera
To find out more, here are the books on ozone published in different languages:
Antonio Gaspari, “Ozone: una cura per la vita”
Antonio Gaspari, “Ozono Solidale: una rivoluzione”
Antonio Gaspari, “Ozono:una cura para la vida”
Read More [fa icon=long-arrow-right"]

DRY EYE More Susceptible to Covid

[fa icon="calendar'] Sep 9, 2021 8:12:04 PM / by Dr. Isadora Guggenheim posted in Autoimmune Disease, Ozone and Prolozone therapies, Corona Virus

[fa icon="comment"] 0 Comments


“An ozone-based ophthalmic gel could act as a barrier against the entry of the SARS CoV-2 virus, thus acting as a sort of mask for the eyes, to be used together with the mask that covers the nose and mouth”.

Click the link to the full article:

This is the idea proposed in a study entitled “COVID-19 Ocular Prophylaxis: The Potential Role of Ozonated-Oils in Liposome Eyedrop Gel”, published in the August issue of Translational Vision Science & Technology:

 The study was directed by Dr. Stanislao Rizzo, Full Professor of Ophthalmology at the Catholic University of the Sacred Heart, Rome campus, and Director of Ophthalmology of the Agostino Gemelli IRCCS University Hospital Foundation.

 “The ozone-based ophthalmic gels – said prof. Rizzo – are already used to relieve the symptoms of inflammatory eye diseases, including those of infectious origin. Our study suggests that they may also have a barrier effect against viruses that enter the eye or nasolacrimal system”.

“This is an in vitro study and the results will have to be confirmed by studies on animal models and humans,” said Rizzo.

 Learn about I.V. Therapies for dry eye, macular degeneration, cataracts and thyroid eye disease. We have treatment protocols. 

In a note, the IRCCS of the Gemelli Polyclinic explained that the SARS CoV-2 virus can also enter the body through the eyes.

In this regard, the study related what happened to a member of the US National Expert Panel on pneumonia, sent to Wuhan for inspection.

The man, who wore no eye protection, first developed conjunctivitis and then the classic Covid-19 pneumonia.

Other studies have shown that the virus can remain in the eye for several weeks after the acute phase of the disease.

 It was therefore discovered that the eyes are a target organ of SARS CoV-2, and that especially those suffering from dry eye syndrome are much more likely to be infected. Yes which human tissue requires the most oxygen? 

Answer: The Retina 

Aging begins in the eyes. That is why you must give your eyes special attention. Get regular eye exams yearly and especially after you have Covid or the vaccine. 

It was thus thought to enhance the barrier effect of tears against SARS CoV-2 by using a special ozone-based gel.

 In the note from the IRCCS of Gemelli it is specified that “ozone has long been used in medicine to disinfect and treat infectious diseases, given its ability to inactivate bacteria, viruses, fungi, yeasts and even protozoa. The antiviral activity of ozone occurs by damaging the viral capsid and altering replication through peroxidation”.

When asked how to spread ozone on the surface of the eye, prof. Rizzo replied that “ozone is very unstable, but to make it suitable for topical use, it can be stabilized by making it react with a monounsaturated fatty acid such as oleic acid to create the so-called ozonated oils, which retain the same properties gaseous ozone and are well tolerated by the tissues.”

“Ozonated oils – he added – are already used in the form of eye drops or ophthalmic gels to relieve the symptoms of inflammatory ocular diseases, including those of infectious origin; ozone also facilitates the healing of corneal damage by minimizing the risk of scarring and opacification of the cornea.”

“The objective of the study – explains prof. Rizzo – is to evaluate whether this eye gel can prevent SARS CoV-2 infection in the tissues of the eye, and also to evaluate whether, in patients with dry eye syndrome, ozone can help restore the integrity of the ocular surface, repairing and regenerating the conjunctival microvilli.”

The in vitro study provided positive results, demonstrating that the ozone-based ophthalmic gel inhibited viral replication and the entry of the virus into the cells on the surface of the eye (corneal and conjunctival).

 It has also been shown that ozone eye gel restores cell regeneration and controls inflammation in dry eye syndrome.

In conclusion, considering that the ocular surface is a potential route of entry for the virus, if the results of this research were to be confirmed by studies on animal models and on humans, it would be conceivable to propose the topical administration of ozone-based ophthalmic gels to prevent the entry of SARS CoV-2 through the ocular surface.

The Director of the Gemelli Ophthalmology Unit believes that the ozone gel is “a protective measure to be recommended first of all to health professionals, and then to be extended to the entire population.”

Keep your eyes out for more on this topic. Medical ozone in several applications works. 

The scientific work was carried out by the researchers: Stanislao Rizzo, Maria Cristina Savastano, Alfonso Savastano, Gloria Gambini (Ophthalmology Unit, A. Gemelli IRCCS Polyclinic Foundation, Catholic University of the Sacred Heart); Daria Bortolotti, Valentina Gentili, Roberta Rizzo (University of Ferrara, Department of Chemical, Pharmaceutical and Agricultural Sciences); Francesca Caccuri (Department of Microbiology and Virology, Spedali Civili, Brescia).


Orbisphera editorial staff

Read More [fa icon=long-arrow-right"]

ALIVE - Ozone Therapy

[fa icon="calendar'] Sep 6, 2021 11:04:00 AM / by Dr. Isadora Guggenheim posted in Ozone and Prolozone therapies

[fa icon="comment"] 0 Comments

From an ozone colleague who followed this story from beginning to end 
Read More [fa icon=long-arrow-right"]

10 Signs You Need Ozone Therapies Now

[fa icon="calendar'] Sep 3, 2021 1:54:40 PM / by Dr. Isadora Guggenheim posted in Ozone and Prolozone therapies

[fa icon="comment"] 0 Comments

Like the line in Harry and Sally, "I'll have what she's having."  She probably had ozone therapy. 

Read More [fa icon=long-arrow-right"]

Secrets to a Healthy Immune System

[fa icon="calendar'] Sep 1, 2021 8:24:38 AM / by Isadora Guggenheim posted in Ozone and Prolozone therapies

[fa icon="comment"] 0 Comments

Listen to what some are saying is a safe and effective therapy for creating a healthy immune system. 

Read More [fa icon=long-arrow-right"]

Chronic Pain - Destroys Your Immune System

[fa icon="calendar'] Aug 31, 2021 8:08:41 AM / by Isadora Guggenheim & Winter Ninivaggi posted in Ozone and Prolozone therapies

[fa icon="comment"] 0 Comments

Chronic pain could be re-programming the way genes work in your immune system. 

Read More [fa icon=long-arrow-right"]

Ozone in Israel

[fa icon="calendar'] Aug 16, 2021 8:31:52 AM / by Dr. Isadora Guggenheim posted in Ozone and Prolozone therapies

[fa icon="comment"] 0 Comments



“With this study we have shown that ozone reduces the infectivity of coronaviruses with a 99% decrease recorded after 30 minutes of exposure to 1,000 ppm of ozone. (...) In the end, we verified that ozone can penetrate every part of a room, proving to be an effective disinfectant to eradicate SARS-CoV-2”. 
We are Ozone experts. Schedule your new patient consult today. We offer I.V. Ozone MAH, ozonated saline, homotoxicology remedies, insufflation and more. 
These are the conclusions of a study conducted by researchers from the Tel Aviv University School of Mechanical Engineering in collaboration with the Materials Engineering Department of the Azrieli College of Engineering in Jerusalem and the Faculty of Medicine of the Bar Ilan University of Safed (Israel). 
The scientific work, published on January 13, 2021 in   Environmental Chemistry Letters, therefore confirms that ozone is a safe and powerful disinfectant against the SARS-CoV-2 virus: 
The researchers were able to experience that, thanks to the use of ozone, the viral infection was reduced by a percentage of about 95% after exposure to ozone for 20 minutes at 1,000 ppm, for 30 minutes at 100 ppm., and for 40 minutes at 30 ppm. 
These trials provide evidence that ozone is extremely effective in deactivating the coronavirus even when used at low concentrations. 
The researchers also highlighted that the advantage offered by ozone over liquid disinfectants consists in the fact that, thanks to its nature as a gas, it is able to penetrate all environments, sanitizing contaminated surfaces. 
Due to its gaseous form, ozone is able to disinfect all surfaces, even in the lateral, lower and internal parts, whereas liquid disinfectants are active only on the surface parts that they can reach directly. 
In this regard, Dr. Ines Zucker of the School of Mechanical Engineering of the University of Tel Aviv and first author of the study, specified that “the advantage of ozone over common disinfectants (such as alcohol and bleach) depends on its ability to disinfect objects and the entire environment inside a room, and not just the exposed surfaces.” 
Dr. Zucker also stressed that “ozone disinfects quickly and without danger to public health because gas can be produced relatively cheaply and easily from oxygen by electric discharge. For this reason, it should be possible to introduce industrial-scale ozone disinfection systems to combat the Covid-19 epidemic.” 
The Israeli study constitutes further confirmation of what has already been demonstrated by the research group of the Faculty of Medicine of the University of Nara in Japan, directed by prof. Toshikazu Yano of the Department of Infectious and Pathogenic Diseases, and by prof. Kei Kasahara of the Center for Infectious Diseases. The experiments of the Japanese scientists – repeated several times – have shown that ozone, spread in environments without the presence of people, is able to permanently deactivate and destroy Covid-19: 
Even more interesting is the experiment conducted, also in Japan, by medical researchers from Fujita Health University (Toyoake, Aichi prefecture), according to which ozone is able to deactivate the coronavirus in environments even if spread in low concentrations. 
Following this discovery, Fujita Medical University Hospital has installed ozone generators to eliminate Covid in waiting rooms and in patient recovery rooms: 
In this context, the Department of Molecular Medicine of the University of Padua, directed by prof. Andrea Crisanti, with Dr. Claudia Del Vecchio as scientific director, conducted an experiment in the Level 3 Biosafety Laboratory. The environment was contaminated with a viral suspension of SARS-CoV-2, after which an ozone producing device of the type used for sanitizing environments. In the end, as reported by the “Virucidal efficacy report”, ozone was able to break down Covid with an effectiveness of 99.98 percent: 
At the beginning of the pandemic, health authorities, while recognizing the virucidal qualities of ozone, remained hesitant, arguing that there was a lack of specific studies capable of confirming the effectiveness of ozone against Covid-19. 
But now – after four university centers around the world have demonstrated how effective, safe and economical is the use of ozone for the sanitization of hospitals, waiting rooms, clinics, schools, means of transport, bars, restaurants, theaters and the like, there appears good reason to aggressively use ozone to interrupt the chain of spread of the virus.
Antonio Gaspari
Director Orbisphera
To learn more, read the book:
Antonio Gaspari, “Ozone: a cure for life”
Read More [fa icon=long-arrow-right"]
Isadora Guggenheim, ND, FNP, RN, MS, CNS, LMT, owner of Second Nature Naturopathic Care, LLC
For all appointments: Tel: 845 358-8385 Fax: 845 358-2963